372
372 .
HALOBACTERIA MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Alkalibacillus silvisoli DSM 18495
* Modification: With 0.2g MgSO4x7H20 only. After sterilization add sterile Na-sesquicarbonate solution (1M) to achieve pH 9.5 (see medium 31)
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C
Main sol. 372 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(Fisher BioReagents BP1422) |
5.00 | g | 5 | - | |
| Casamino acids | 5.00 | g | 5 | - | |
| Na glutamate | 1.00 | g | 1 | 5.913 | |
| KCl | 2.00 | g | 2 | 26.828 | |
| Na3-citrate | 3.00 | g | 3 | 11.625 | |
| MgSO4 x 7 H2O* | 0.20 | g/l | - | - | |
| NaCl | 200.00 | g | 200 | 3422.31 | |
| FeCl2 x 4 H2O | 36.00 | mg | 0.036 | 0.181 | |
| MnCl2 x 4 H2O | 0.36 | mg | 3.6e-4 | 0.002 | |
| Agar | 20.00 | g | 20 | 59.465 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add distilled water to give a final volume of 1000 ml. | |||||
| 2 Adjust pH to 7.0 - 7.2 | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | * 9.5 |
| Edit in Medium builder | |
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 200 | 3422.31 |
| MgSO4 x 7 H2O | 20 | 81.144 |
| Agar | 20 | 59.465 |
| Yeast extract | 5 | - |
| Casamino acids | 5 | - |
| Show more | ||
Cultivation metadata from 111 strains
| halophily | moderately halophilic | 3 strains |
| halophily | extremely halophilic | 2 strains |
| pH | 8 - 10 | 21 strains |
| pH | 6 - 8 | 19 strains |
| pH | 4 - 6 | 6 strains |
| Show more | ||